Investigation of major mutagens in airborne particulate matter

Thesis/Dissertation · Wed Jan 01 04:00:00 EST 1992

OSTI ID:5571539

Lwo, J

The separation of complex environmental mixtures in order to identify bioactive substances usually involves a multi-level scheme culminating in detailed chemical analysis. The approach of this research program involves a fractionation scheme which includes acid/base/neutral partitioning, silica gel column chromatography and two-stage HPLC separation. This separation scheme has been applied in the bioassay-directed search for major airborne mutagenic compounds on inhalable particulate matter. In other words, the biologically [open quotes]hot[close quotes] fractions are separated and analyzed chemically or subfractionated to isolate [open quotes]hot[close quotes] subfractions which are then chemically analyzed by gas chromatography/mass spectrometry (GC/MS), gas chromatography/negative chemical ionization mass spectrometry (GC/NCIMS), Fourier transform infrared (FTIR), high performance liquid chromatography (HPLC), Fluorescence and Diode Array UV. The mutagenicity assay involves the inactivated TA98 Salmonella strain (TA98-S9) as well as enzyme-activated (TA98+S9) strain. Some other assays containing TA98NR (TA98-nitroreductase deficient), TA98/1,8DNP[sub 6] (TA98-dinitropyrene reductase deficient) and TA100+/-S9 strains have been also performed to detect the highly bioactive derivatives of polycyclic aromatic hydrocarbons (PAH). In essence, we are employing mutagenicity as our chromatographic detector to pinpoint the most bioactive fractions and compounds in the air which are assumed to be responsible for human health impact, and then identify them as well as assess their reactivity. The comparison of winter, summer and fall samples indicates that the profiles are similar in these three periods.

• Research Organization: Rutgers--the State Univ., New Brunswick, NJ (United States)
• OSTI ID: 5571539
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540120* -- Environment
Atmospheric-- Chemicals Monitoring & Transport-- (1990-)
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AIR POLLUTION
AIR QUALITY
BIOLOGICAL INDICATORS
ENVIRONMENTAL EXPOSURE
ENVIRONMENTAL QUALITY
HAZARDS
HEALTH HAZARDS
MULTI-PARAMETER ANALYSIS
MUTAGEN SCREENING
MUTAGENS
PARTICLES
PARTICULATES
POLLUTION
SCREENING
SEASONAL VARIATIONS
VARIATIONS