S9-activated Ames assays of diesel-particle extracts. Detecting indirect-acting mutagens in samples that are direct-acting
- Ford Motor Co., Dearborn, MI (USA)
The authors sought to detect S9-activated mutagens in diesel-exhaust particle extracts by fractionating extracts using high-performance liquid chromatography (HPLC) and carrying out Ames assays (strains TA98 and TA100) with and without S9 mix on the eight fractions obtained thereby. S9 activation of the fraction containing unsubstituted and alkyl-substituted polycyclic aromatic hydrocarbons (PAH) was at the limits of detectability for strain TA100 and undetectable for TA98. There was only one fraction that showed S9 activation; it was 20-fold more active with S9 mix. Six other fractions were strongly direct-acting and showed no enhancement of activity with S9 mix. The S9-activated mutagens detected when concentrated in a chromatographic fraction could not be detected in unfractionated extracts because the direct-acting mutagenicity of the other components dominated. They were unable to identify the indirect-acting mutagens from GC-MS data.
- OSTI ID:
- 5457706
- Journal Information:
- Environmental Science and Technology; (United States), Journal Name: Environmental Science and Technology; (United States) Vol. 24:6; ISSN ESTHA; ISSN 0013-936X
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
CHROMATOGRAPHY
DIESEL ENGINES
ENGINES
EXHAUST GASES
FLUIDS
GAS CHROMATOGRAPHY
GASEOUS WASTES
GASES
HEAT ENGINES
HYDROCARBONS
INTERNAL COMBUSTION ENGINES
LIQUID COLUMN CHROMATOGRAPHY
MASS SPECTROSCOPY
MUTAGEN SCREENING
ORGANIC COMPOUNDS
PARTICLES
PARTICULATES
POLYCYCLIC AROMATIC HYDROCARBONS
SCREENING
SEPARATION PROCESSES
SPECTROSCOPY
WASTES