Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Ames assay chromatograms and the identification of mutagens in diesel particle extract

Journal Article · · Environ. Sci. Technol.; (United States)
DOI:https://doi.org/10.1021/es00123a017· OSTI ID:7012674
; ; ; ;

Dichloromethane extracts of passenger car diesel exhaust particles were separated into 65 fractions by normal-phase high-performance liquid chromatography (HPLC), and Ames assays (TA98 without activation) were carried out on each fraction. The only prominently mutagenic fractions were ones with elution times approximately coincident with those for 1,3-, 1,6-, and 1,8-dinitropyrenes, 1-nitropyrene, and 3- and 8-nitrofluoranthene. These compounds were quantified in one extract sample by gas chromatography-mass spectrometry and gas chromatography coupled with a nitrogen-phosphorus detector. Among five extract samples studied, 30-40% of the total mutagenicity recovered from the columns could be attributed to these six nitroarenes, 15-20% was recovered in the as yet uncharacterized polar fraction, and the remainder was distributed roughly equally over 63 fractions. About 70% of the mutagenicity applied to the HPLC columns was recovered from the columns. The Ames assay mutagenicities of the unfractionated extracts were shown to be additive in the mutagenicities of the components. 23 references.

Research Organization:
Ford Motor Co., Dearborn, MI
OSTI ID:
7012674
Journal Information:
Environ. Sci. Technol.; (United States), Journal Name: Environ. Sci. Technol.; (United States) Vol. 18:5; ISSN ESTHA
Country of Publication:
United States
Language:
English

Similar Records

S9-activated Ames assays of diesel-particle extracts. Detecting indirect-acting mutagens in samples that are direct-acting
Journal Article · Fri Jun 01 00:00:00 EDT 1990 · Environmental Science and Technology; (United States) · OSTI ID:5457706
Dinitro and mononitrobenzo(ghi)perylenes and mononitrocoronene are highly mutagenic in the Ames Salmonella assay
Journal Article · Fri Dec 31 23:00:00 EST 1982 · Environ. Manage.; (United States) · OSTI ID:5073723
Purified rat liver DT-diaphorase potentiates the mutagenicity of 1,3-, 1,6- and 1,8-dinitropyrene
Conference · Sun Mar 10 23:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States) · OSTI ID:5863333

Related Subjects

560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AROMATICS
AUTOMOBILES
BACTERIA
CHROMATOGRAPHY
CONDENSED AROMATICS
DIESEL FUELS
EXHAUST GASES
FLUIDS
FRACTIONATION
GAS CHROMATOGRAPHY
GASEOUS WASTES
GASES
HYDROCARBONS
LIQUID COLUMN CHROMATOGRAPHY
MASS SPECTROSCOPY
MICROORGANISMS
MUTAGEN SCREENING
MUTAGENESIS
NITRO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARTICLES
PARTICULATES
PETROLEUM PRODUCTS
PYRENE
SALMONELLA
SCREENING
SEPARATION PROCESSES
SPECTROSCOPY
VEHICLES
WASTES