Purified rat liver DT-diaphorase potentiates the mutagenicity of 1,3-, 1,6- and 1,8-dinitropyrene
- Louisiana State Univ., Baton Rouge (United States)
The effect of highly purified rat liver cytosolic DT-diaphorase on the mutagenicity of 1,3-, 1,6- and 1,8-dinitropyrene (DNP) was studied in the Ames Salmonella typhimurium mutagenicity assay. DT-diaphorase over the range of 0 - 0.78 {mu}g/plate increased the mutagenicity of all three DNP in S. typhimurium TA 98 to a maximum of 3-fold. In the nitroreductase-deficient strain, TA98NR, 1,6- and 1,8-DNP were activated to a lesser extent in the presence of equal amounts of DT-diaphorase as compared to TA98. The mutagenicity of 1,3-DNP was markedly lowered in TA98NR and DT-diaphorase did not increase the mutagenicity of this toxicant except at the highest level utilized. DT-diaphorase was found to activate 1,6-, but not 1,3-DNP to mutagenic intermediates in TA98 18DNP{sub 6}, a strain deficient in O-acetyltransferase activity. The results suggest that DT-diaphorase not only catalyzes reduction of the parent DNP but also that of partially reduced metabolites generated from that DNP. This may result in increased formation of the penultimate mutagenic species. Thus, while DT-diaphorase is commonly viewed as having antimutagenic properties with certain compounds the opposite appears to be true for DNP.
- OSTI ID:
- 5863333
- Report Number(s):
- CONF-9104107--
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Vol. 5:4; ISSN FAJOE; ISSN 0892-6638
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BACTERIA
BODY
DIGESTIVE SYSTEM
ENZYME ACTIVITY
ENZYMES
GLANDS
LIVER
MAMMALS
METABOLIC ACTIVATION
MICROORGANISMS
MUTAGEN SCREENING
NITRO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDOREDUCTASES
PROTEINS
RATS
RODENTS
SALMONELLA
SALMONELLA TYPHIMURIUM
SCREENING
VERTEBRATES