Detection of nucleic acid hybrids by prolonged chemiluminescence

Patent · Tue Dec 27 04:00:00 EST 1988

OSTI ID:6292558

Dattagupta, N; Clemens, A H

A method for determining a particular single stranded polynucleotide sequence in a test medium, comprising the steps of: (a) immobilizing on a solid support single stranded nucleic acids in the test medium, (b) contacting the immobilized nucleic acids with a polynucleotide probe having a base sequence substantially complementary to the sequence to be determined and the contacting being under conditions favorable to hybridization between the probe and the sequence to be determined, wherein the probe is labeled with a chemiluminescence enhancer, (c) separating the immobilized hybrids from the unhybridized probe, (d) initiating a chemiluminescent reaction by contacting the separated, labeled, immobilized hybrids with an oxidant, a 2.3-dihydro-1,4-phthalazinedione chemiluminescence precursor, and a peroxidase enzyme, (e) detecting the resulting light emission, and (f) relating the amount of emitted light to the amount of the single stranded polynucleotide sequence.

• Assignee: Molecular Diagnostics, Inc., West Haven, CT
• Patent Number(s): US 4794073
• OSTI ID: 6292558
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
CHEMICAL COMPOSITION
CHEMILUMINESCENCE
DETECTION
HYBRIDIZATION
IMMOBILIZED ENZYMES
LABELLING
LUCIFERIN
LUMINESCENCE
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
OXIDIZERS
PRECURSOR
PROTEINS
SEPARATION PROCESSES
VITRIFICATION