Solution-phase single hybridization assay for detecting polynucleotide sequences
Patent
·
OSTI ID:5060149
This patent describes a process for determining the presence of a particular nucleic acid sequence in a sample containing cells. It comprises chemically modifying nucleic acids in an unpurified sample; conducting hybridization; contacting under hybridization conditions the chemically modified sample nucleic acids with a hybridizable nucleic acid probe; contacting the solution with an immobilized form of a reactive site on the sample nucleic acids or the probe, respectively; separating the resulting immobilized fraction from the remaining solution; and determining the presence of the label in the separated immobilized fraction or a decrease in the label in the remaining solution.
- Assignee:
- Molecular Diagnostics, Inc., West Haven, CT (USA)
- Patent Number(s):
- US 4968602; A
- Application Number:
- PPN: US 7-442423
- OSTI ID:
- 5060149
- Country of Publication:
- United States
- Language:
- English
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