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Detection and isolation of nucleic acid sequences using competitive hybridization probes

Patent ·
OSTI ID:870890
 [1];  [2];  [3]
  1. San Ramon, CA
  2. Tracy, CA
  3. Walnut Creek, CA
+ Show Author Affiliations

A method for detecting a target nucleic acid sequence in a sample is provided using hybridization probes which competitively hybridize to a target nucleic acid. According to the method, a target nucleic acid sequence is hybridized to first and second hybridization probes which are complementary to overlapping portions of the target nucleic acid sequence, the first hybridization probe including a first complexing agent capable of forming a binding pair with a second complexing agent and the second hybridization probe including a detectable marker. The first complexing agent attached to the first hybridization probe is contacted with a second complexing agent, the second complexing agent being attached to a solid support such that when the first and second complexing agents are attached, target nucleic acid sequences hybridized to the first hybridization probe become immobilized on to the solid support. The immobilized target nucleic acids are then separated and detected by detecting the detectable marker attached to the second hybridization probe. A kit for performing the method is also provided.

Research Organization:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA
DOE Contract Number:
W-7405-ENG-48
Assignee:
Regents of University of California (Oakland, CA)
Patent Number(s):
US 5616465
OSTI ID:
870890
Country of Publication:
United States
Language:
English

References (3)

Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes. journal November 1981
Ligation of oligonucleotides to nucleic acids or proteins via disulfide bonds journal May 1988
Gene mapping and gene enrichment by the avidin-biotin interaction: use of cytochrome-c as a polyamine bridge journal January 1978

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