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Title: Detection and isolation of nucleic acid sequences using a bifunctional hybridization probe

Patent ·
OSTI ID:872867
 [1];  [2];  [3]
  1. San Ramon, CA
  2. Tracy, CA
  3. Walnut Creek, CA
+ Show Author Affiliations

A method for detecting and isolating a target sequence in a sample of nucleic acids is provided using a bifunctional hybridization probe capable of hybridizing to the target sequence that includes a detectable marker and a first complexing agent capable of forming a binding pair with a second complexing agent. A kit is also provided for detecting a target sequence in a sample of nucleic acids using a bifunctional hybridization probe according to this method.

Research Organization:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
DOE Contract Number:
W-7405-ENG-48
Assignee:
Regents of University of California (Oakland, CA)
Patent Number(s):
US 6027879
OSTI ID:
872867
Country of Publication:
United States
Language:
English

References (3)

Ligation of oligonucleotides to nucleic acids or proteins via disulfide bonds journal May 1988
Gene mapping and gene enrichment by the avidin-biotin interaction: use of cytochrome-c as a polyamine bridge journal January 1978
Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes. journal November 1981

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detection
isolation
nucleic
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bifunctional
hybridization
probe
method
detecting
isolating
target
sequence
sample
acids
provided
capable
hybridizing
detectable
marker
complexing
agent
forming
binding
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kit
according
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acid sequence
nucleic acid
acid sequences
nucleic acids
target sequence
complexing agent
hybridization probe
agent capable
bifunctional hybridization
probe capable
binding pair
/435/999/