Nucleic acid hybridization assays
This patent describes a method for carrying out a nucleic acid hybridization test to detect a target nucleic acid sequence. It comprises: providing a polymer molecule bonded to a first single stranded nucleic acid sequence comprising a first probe which hybridizes to a first target nucleic acid sequence when present, provided that, where the polymer molecule comprises DNA, the DNA is of heterogeneous base sequence; denaturing the target nucleic acid sequence; contacting the denatured target nucleic acid sequence with the polymer molecule bonded to the first single stranded nucleic acid sequence; separating out complexes between the target nucleic acid sequence and the polymer molecule by trapping the complexes on a two-dimensional surface; and detecting complexes between the target nucleic acid sequence and the polymer molecule.
- Assignee:
- Biotechnica Diagnostics, Inc., Cambridge, MA (USA)
- Patent Number(s):
- A; US 4925785
- Application Number:
- PPN: US 6-837701A
- OSTI ID:
- 6719852
- Country of Publication:
- United States
- Language:
- English
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