Method for increasing the sensitivity of nucleic acid hybridization assays
Patent
·
OSTI ID:5934943
A hybridization assay method is described for detecting a specific polynucleotide target sequence wherein a nucleic acid containing target sample in single stranded form is affixed to a support. It is contacted thereon under hybridizing conditions with a non-radioactive reporter group labeled single-stranded polynucleotide probe having a sequence complementary to the target sequence, the probe being bound to the affixed sample when the target is present. The probe hybridizer with the target sequence, then the unbound portion of the probe is removed from the support, and the presence of the probe is detected by means of its reporter group, wherein the sensitivity of the assay is increased by employing the additional steps comprising: (a) after removal of the unbound portion of the probe, dehybridizing the bound portion; (b) concentrating the dehybridized separated probe onto a solid support; and (c) detecting the presence of the probe on the solid support by means of the non-radioactive reporter group.
- Assignee:
- Molecular Biosystems, Inc., San Diego, CA
- Patent Number(s):
- US 4824776
- OSTI ID:
- 5934943
- Country of Publication:
- United States
- Language:
- English
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