Methods for attaching polynucleotides to supports
Patent
·
OSTI ID:6152748
A method is described for a hybridization assay comprising the steps of: attaching a first single-stranded polynucleotide to a support so that the first single-stranded polynucleotide has a first free end; hybridizing a first portion of a second single-stranded polynucleotide with the first single-stranded polynucleotide so that the second single-stranded polynucleotide has a free single-stranded end; connecting by hybridization a third single-stranded polynucleotide, so that the third single-stranded polynucleotide has a first end adjacent the first end of the first single-stranded polynucleotide; ligating the first end of the first polynucleotide to the first end of the third polynucleotide to form a probe polynucleotide bound to the support; denaturing the second single-stranded polynucleotide from the probe polynucleotide; and immobilizing a fourth polynucleotide constituting a target analyte by hybridization to the probe polynucleotide.
- Assignee:
- Amgen, Inc., Thousand Oaks, CA
- Patent Number(s):
- US 4797355
- OSTI ID:
- 6152748
- Country of Publication:
- United States
- Language:
- English
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