Detection of specific sequences in nucleic acids

Patent · Tue Nov 28 04:00:00 EST 1989

OSTI ID:6999491

Whiteley, N M; Hunkapiller, M N; Glazer, A N

This patent describes a method of testing for the presence or absence of a target sequence in a sample containing DNA. The steps comprising: denaturing the sample DNA; treating the denatured DNA with a first probe which is complementary to a first portion of the target sequence to produce a duplex of first probe and denatured DNA; treating the duplex formed with a second probe, which is shorter than the first probe and ligatable therewith, and which is complementary to the remaining portion of the target sequence; providing conditions for hybridization of the first probe and second probe to the target sequence in the second two steps wherein, by controlling stringency, the first probe does not bind substantially to the denature DNA which does not contain the first portion and wherein the second probe does not bind substantially to the denatured DNA which does not contain the remaining portion, the first probe and the second probe being of sufficient length that they can be stably hybridized to the target sequence long enough to be ligated to each other, and wherein at least one of the first and second probe is labeled; ligating first and second probe which are hybridized to the target sequence; removing unligated probe; testing for the presence of label.

• Assignee: Applied Biosystems, Inc., Foster City, CA
• Patent Number(s): A; US 4883750
• Application Number: PPN: US 7-681055A
• OSTI ID: 6999491
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
DNA SEQUENCING
HYBRIDIZATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
STRUCTURAL CHEMICAL ANALYSIS
TESTING