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Changes in GDP binding to brown adipose tissue mitochondria and the uncoupling protein

Journal Article · · American Journal of Physiology; (USA)
OSTI ID:6289488
;  [1]
  1. Univ. of Wisconsin, Madison (USA)
+ Show Author Affiliations
Incubation in vitro of brown adipose tissue (BAT) mitochondria with divalent cations, spermine, or alkaline phosphatase led to a marked increase in the binding of ({sup 3}H)GDP. The effect of Mg{sup 2+} appeared to be the most specific and led to the largest increase in GDP binding. A simplified method was developed for measuring GDP binding to purified uncoupling protein from rat BAT mitochondria. Application of this method indicates that uncoupling protein from cold-acclimated rats binds twice as much GDP as uncoupling protein from cold-acclimated rats that were briefly returned to thermoneutrality, paralleling changes in GDP binding to the mitochondria. Incubation of BAT mitochondria with Mg{sup 2+} led to a smaller increase in GDP binding to the subsequently purified uncoupling protein, suggesting that divalent cations may somehow participate in the regulation of the activity of the uncoupling protein.
OSTI ID:
6289488
Journal Information:
American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 255:6; ISSN 0002-9513; ISSN AJPHA
Country of Publication:
United States
Language:
English

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Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADIPOSE TISSUE
ALKALINE EARTH METAL COMPOUNDS
ALKALINE PHOSPHATASE
ANIMAL TISSUES
BIOLOGICAL EFFECTS
BODY
CALCIUM COMPOUNDS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CATIONS
CELL CONSTITUENTS
CHARGED PARTICLES
CHEMICAL ACTIVATION
CHEMICAL REACTIONS
CONNECTIVE TISSUE
CROSS-LINKING
DISACCHARIDES
ENZYMES
ESTERASES
HYDROGEN COMPOUNDS
HYDROLASES
IONS
ISOTOPE RATIO
LABELLED COMPOUNDS
MAGNESIUM COMPOUNDS
MEMBRANE PROTEINS
MITOCHONDRIA
NUCLEOTIDES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANOIDS
PHOSPHATASES
POLYMERIZATION
PROTEINS
RECEPTORS
SACCHARIDES
SACCHAROSE
TISSUES
TRITIUM COMPOUNDS