Concentration of rat brown adipose tissue uncoupling protein may not be correlated with /sup 3/H-GDP binding

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5393060

Henningfield, M F; Swick, A G; Swick, R W

Rats fed diets low in protein or exposed to cold show an increase in brown adipose tissue (BAT) mitochondrial /sup 3/H-GDP binding. To investigate this phenomenon further, the uncoupling protein associated with BAT function was measured immunochemically on nitrocellulose blots. Quantitation of uncoupling protein was achieved by densitometer scanning with a BioRad densitometer. Peaks were integrated with Chromatochart software and an Apple IIe computer. A standard curve of purified uncoupling protein (50 to 500 ng) was used to calculate uncoupling protein concentration. There is a 1.5-fold increase in uncoupling protein per mg of protein in BAT mitochondria from rats exposed to cold for 15 days. There was no decrease in uncoupling protein from rats exposed to the cold followed by 24 h at 27/sup 0/C although /sup 3/H-GDP binding had decreased by half. Rats fed diets containing either 5 or 15% lactalbumin for 3 weeks did not show differences in uncoupling protein concentration although /sup 3/H-GDP binding was 1.5-fold greater in BAT mitochondria from the low protein group. These results indicate that GDP binding does not necessarily reflect the concentration of uncoupling protein in BAT mitochondria.

• Research Organization: Univ. of Wisconsin, Madison
• OSTI ID: 5393060
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADIPOSE TISSUE
ANIMAL TISSUES
ANIMALS
AROMATICS
AZAARENES
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
BIOLOGICAL ACCUMULATION
BODY
CHEMISTRY
COMPUTER CALCULATIONS
CONNECTIVE TISSUE
GUANOSINE
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
PURINES
RATS
REACTION KINETICS
RIBOSIDES
RODENTS
TEMPERATURE EFFECTS
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES