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Rapid changes in number of GDP binding sites on brown adipose tissue mitochondria

Journal Article · · Am. J. Physiol.; (United States)
OSTI ID:6281030
;
(TH)GDP binding to brown adipose tissue (BAT) mitochondria increased more than twofold in 20 min when rats were moved from 27 to 4C. When animals housed at 4C for 2 h were returned to 27C, GDP binding decreased sharply in 20 min and returned to control levels in 2 h. These results are consistent with a rapid unmasking and remasking of GDP bindings sites. GDP binding to mitochondria from warm and acutely cold treated rats was not modified by prior swelling, by freeze-thawing, nor by sonication of the mitochondria before assay. GDP-inhibitable proton conductance, as measured by passive swelling, was unaffected by this brief exposure to cold but more than doubled in rats kept at 4C for 10 days. The authors hypothesize that the rate of GDP-inhibitable swelling may be a reflection of uncoupling protein concentration in the BAT mitochondria, whereas physiological thermogenic activity is more appropriately indicated by GDP binding. The alterations in binding activity appear not to be due to changes in the mitochondrial membrane integrity.
Research Organization:
Univ. of Wisconsin, Madison
OSTI ID:
6281030
Journal Information:
Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 251:2; ISSN AJPHA
Country of Publication:
United States
Language:
English

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Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADIPOSE TISSUE
ANIMAL TISSUES
ANIMALS
AROMATICS
AZAARENES
BODY
CARBON 14 COMPOUNDS
CELL CONSTITUENTS
CONFIGURATION INTERACTION
CONNECTIVE TISSUE
GUANOSINE
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MITOCHONDRIA
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
PHYSIOLOGY
PURINES
RATS
RESPIRATION
RIBOSIDES
RODENTS
TEMPERATURE DEPENDENCE
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES