Ozone effect on lysosomal hydrolases of alveolar macrophages in vitro

Journal Article · Thu Dec 31 23:00:00 EST 1970 · Arch. Intern. Med.; (United States)

DOI:https://doi.org/10.1001/archinte.127.6.1059· OSTI ID:6288825

Hurst, D J; Coffin, D L

Rabbit alveolar macrophages in thin-layer tissue culture were exposed in vitro to 2 ppM O/sub 3/ for 3 hr. Slight reduction (approx. 15%) in macrophages adhering to culture after 3-hr exposure was noted. There was a decrease in intracellular hydrolytic enzymes (acid phosphatase, lysozyme, and ..beta..-glucuronidase) but an increase in extracellular activity indicating a loss through membrane. Totals never add up to original, so ozone may also cause some inactivation. A concentration of 2.5 ..mu..mole/ml glutathione or cysteine prevented loss of hydrolytic enzyme activity; 1.5 ..mu..mole was not as good and 0.5 ..mu..mole had little effect.

• OSTI ID: 6288825
• Journal Information: Arch. Intern. Med.; (United States), Journal Name: Arch. Intern. Med.; (United States) Vol. 127; ISSN AIMDA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACID PHOSPHATASE
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BIOCHEMICAL REACTION KINETICS
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CONNECTIVE TISSUE CELLS
CYSTEINE
ENZYMES
ESTERASES
GLUCURONIDASE
GLUTATHIONE
GLYCOSYL HYDROLASES
HYDROLASES
KINETICS
LUNGS
LYSOSOMES
LYSOZYME
MACROPHAGES
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANOIDS
ORGANS
OZONE
PEPTIDES
PHAGOCYTES
PHOSPHATASES
POLYPEPTIDES
PROTEINS
RABBITS
REACTION KINETICS
RESPIRATORY SYSTEM
SOMATIC CELLS
THIOLS
TISSUE CULTURES
TOXICITY
VERTEBRATES