Radioisotopic model for investigating thromboembolism in the rabbit

Journal Article · Wed Aug 01 00:00:00 EDT 1990 · Journal of Pharmacological Methods; (USA)

DOI:https://doi.org/10.1016/0160-5402(90)90046-N· OSTI ID:6282824

May, G R; Herd, C M; Butler, K D; Page, C P ^[1]

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1. King's College London (England)

111Indium ({sup 111}In)-oxine labeled platelets have been used in a variety of species to assess platelet behavior in vivo. We have shown that {sup 111}In-oxine is a suitable label for rabbit platelets and, using a noninvasive technique for the automated, continuous, external imaging of these radiolabeled platelets, we have shown that intravenous adenosine disphosphate (ADP), collagen, platelet activating factor (PAF), and thrombin all elicit dose-related accumulation of platelet-(but not erythrocyte-)associated radioactivity in the thoracic region and a concomitant fall in both the cranial and hindlimb regions of the anesthetized rabbit. Intracarotid (i.c.) ADP, collagen, PAF, and thrombin also produce dose-related increases in platelet-associated radioactivity in the thoracic and decreases in the cranial and hindlimb regions. However, the initial fall in cranial counts induced by i.c. thrombin was followed by a marked increase that was sustained for up to 3 hr. These results suggest this may be a useful model for investigating the mechanisms of platelet activation in the arterial and venous circulations in vivo and may provide a novel model for investigating thromboembolic events in the cerebral circulation.

• OSTI ID: 6282824
• Journal Information: Journal of Pharmacological Methods; (USA), Journal Name: Journal of Pharmacological Methods; (USA) Vol. 24:1; ISSN 0160-5402; ISSN JPMED
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ADP
ANIMALS
AROMATICS
AZAARENES
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL MODELS
BLOOD
BLOOD CELLS
BLOOD COAGULATION FACTORS
BLOOD PLATELETS
BODY FLUIDS
COAGULANTS
COLLAGEN
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
DISEASES
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
HEMATOLOGIC AGENTS
HEMOSTATICS
HETEROCYCLIC COMPOUNDS
HYDROLASES
HYDROXY COMPOUNDS
IMAGE PROCESSING
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXINE
PEPTIDE HYDROLASES
PROCESSING
PROTEINS
PYRIDINES
QUINOLINES
RABBITS
RADIOISOTOPES
SCLEROPROTEINS
SERINE PROTEINASES
THROMBIN
VASCULAR DISEASES
VERTEBRATES