Subcellular localization of /sup 111/In in human and rabbit platelets

Journal Article · Mon Feb 01 04:00:00 EST 1982 · Blood; (United States)

OSTI ID:5550869

Baker, J R; Butler, K D; Eakins, M N; Pay, G F; White, A M

In order to examine the subcellular distribution of /sup 111/In in /sup 111/In-oxine-labeled human and rabbit platelets, we employed a hypothetical grain technique of EM autoradiography analysis. The results indicate that in the rabbit /sup 111/In was concentrated within the platelet dense bodies, particularly when the platelets had been labeled in a plasma-free system. Under comparable conditions of labeling, human platelets appeared to accumulate almost all the radiolabel within the cytosol. Using inhibitors of 5-hydroxytrptamine (5-HT) uptake, i.e., cloimipramine, ouabain, sodium fluoride, p-chloromercuribenzoate, and reserpine, we were unable to demonstrate an active uptake process in either species. Both collagen and thrombin were able to cause dose-dependent release of radioactivity from the labeled rabbit platelets only. In the case of collagen, this mimicked endogenous 5-HT release and was inhibited by indomethacin. These results and their implications are discussed.

• Research Organization: Ciba-Geigy Pharmaceuticals Division, Horsham Research Centre, West Sussex, UK
• OSTI ID: 5550869
• Journal Information: Blood; (United States), Journal Name: Blood; (United States) Vol. 59:2; ISSN BLOOA
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL ACCUMULATION
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CHEMISTRY
COAGULANTS
COLLAGEN
DAYS LIVING RADIOISOTOPES
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ELECTRON MICROSCOPY
ENZYMES
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
INDIUM 111
INDIUM ISOTOPES
INHIBITION
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MAN
MATERIALS
MICROSCOPY
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
PRIMATES
PROTEINS
RABBITS
RADIOISOTOPES
SCLEROPROTEINS
SERINE PROTEINASES
THROMBIN
UPTAKE
VERTEBRATES