Subcellular localization of /sup 111/In in human and rabbit platelets
- Horshan Research Centre, West Sussex, United Kingdom
In order to examine the subcellular distribution of /sup 111/In in /sup 111/In-oxine-labeled human and rabbit platelets, we employed a hypothetical grain technique of EM autoradiograph analysis. The results indicated that in the rabbit /sup 111/In was concentrated within the platelet dense bodies, particularly when the platelets had been labeled in a plasma-free system. under comparable conditions of labeling, human platelets appeared to accumulate almost all the radiolabel within the cytosol. Using inhibitors of 5-hydroxytryptamine (5-HT) uptake, i.e., cloimipramine, ouabain, sodium fluoride, p-chloromercuribenzoate, and reserpine, we were unable to demonstrate an active uptake process in either species. both collagen and thrombin were able to cause dose-dependent release of radioactivity from the labeled rabbit platelets only. In the case of collagen, this mimicked endogenous 5-HT release and as inhibited by indomethacin. These results and their implications are discussed.
- OSTI ID:
- 5734148
- Journal Information:
- Blood; (United States), Journal Name: Blood; (United States) Vol. 59:2; ISSN BLOOA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Subcellular localization of indium-111 in indium-111-labeled platelets
Indium-111-labeled human platelets: uptake and loss of label and in vitro function of labeled platelets
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AUGER EFFECT
AUTORADIOGRAPHY
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CELL CONSTITUENTS
CYTOPLASM
DAYS LIVING RADIOISOTOPES
DOSE-RESPONSE RELATIONSHIPS
ELECTRON CAPTURE RADIOISOTOPES
ELECTRON MICROSCOPY
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MICROSCOPY
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXINE
PYRIDINES
QUINOLINES
RABBITS
RADIOISOTOPES
TRACER TECHNIQUES
VERTEBRATES