Indium-111-labeled human platelets: uptake and loss of label and in vitro function of labeled platelets

Journal Article · Wed Nov 01 04:00:00 EST 1978 · J. Lab. Clin. Med.; (United States)

OSTI ID:6421465

Joist, J H; Baker, R; Thakur, M L; Welch, M J

Indium-111 (/sup 111/In)-oxine has recently been suggested as a new agent for isotopic labeling of platelets. In this paper, the results of in vitro studies on /sup 111/In-oxine uptake by and loss from human platelets as well as function of labeled platelets are presented. /sup 111/In-oxine was rapidly taken up by platelets in a plasma-poor medium, with a mean labeling efficiency of 77% at 37/sup 0/C. Uptake of label was reduced at lower temperatures but did not appear to be dependent on intact platelet energy metabolism. The percentage uptake of /sup 111/In-oxine was found to be similar at concentrations between 1 and 30 ..mu..Ci/ml platelet suspension but was markedly reduced in the presence of small concentrations of plasma proteins. The in vitro sensitivity of /sup 111/In-labeled platelets in response to different aggregating and release-inducing stimuli was similar to that of unlabeled control platelets. Uptake of /sup 111/In-oxine by thrombin-degranulated platelets was similar to that observed with intact platelets, and less than 4% of label was lost from /sup 111/In-labeled platelets upon exposure to strong release-inducing agents. This indicates that the label is bound to nonreleasable platelet components. Less than 3% of label was lost from /sup 111/In-labeled platelets during a 2 hr in vitro incubation at 37/sup 0/C. These in vitro findings are consistent with and support previously reported in vivo evidence indicating that /sup 111/In-oxine may be a suitable and promising new isotopic label for studies of platelet survival, organ distribution, and thrombus localization.

• OSTI ID: 6421465
• Journal Information: J. Lab. Clin. Med.; (United States), Journal Name: J. Lab. Clin. Med.; (United States) Vol. 92:5; ISSN JLCMA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
IN VITRO
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLING
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
RADIOISOTOPES
UPTAKE