Indium-111-labeled human platelets: improved method, efficacy, and evaluation

Journal Article · Wed Apr 01 04:00:00 EST 1981 · J. Nucl. Med.; (United States)

OSTI ID:6462544

Thakur, M L; Walsh, L; Malech, H L; Gottschalk, A

An improved method for labeling human platelets with In-111 oxine is described. The method used modified Tyrode's solution (MTS) as a labeling medium and allowed 86 +- 7% incorporation of In-111 into platelets, which upon stimulation with ADP aggregated 66 +- 15% of control platelets. This compared favorably with the 5% radioactivity uptake in plasma and the 36 and 55% aggregability of platelets labeled in saline and citrated saline, respectively. The influence of parameters affecting the platelet aggregability were examined. The studies revealed that excess of oxine (50 ..mu..g oxine/1.6 x 10/sup 9/ platelets in 1 ml MTS), and centrifugation at greater than 1000 g, reduced platelet aggregability. Excess of oxine also caused a dose-dependent release of In-111 from platelets labeled either in MTS or in plasma, but 4 millimol EDTA did not. Release of In-111 upon aggregation of platelets labeled in either medium was negligible.

• Research Organization: Yale Univ., New Haven, CT
• OSTI ID: 6462544
• Journal Information: J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 22:4; ISSN JNMEA
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400702 -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AGGLOMERATION
AMINO ACIDS
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CARBOXYLIC ACIDS
CHELATING AGENTS
CHEMICAL PREPARATION
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DOSE-RESPONSE RELATIONSHIPS
DRUGS
EDTA
ELECTRON CAPTURE RADIOISOTOPES
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LABELLING
MAMMALS
MAN
MATERIALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXINE
PRIMATES
PYRIDINES
QUINOLINES
RADIOISOTOPE SCANNING
RADIOISOTOPES
RADIOPHARMACEUTICALS
SCINTISCANNING
SYNTHESIS
VERTEBRATES