Indium-111 tropolone, a new tracer for platelet labeling
Platelets have been labeled with a new neutral, lipid-soluble metal complex of indium 111 (/sup 111/In) and tropolone. Unlike oxine, which is soluble in ethyl alcohol, tropolone is soluble in isotonic saline. Platelet labeling with /sup 111/In tropolone can be performed in both acid-citrate-dextrose (ACD) plasma and ACD saline within two hours. Labeling efficiency has been 80% to 90%. /sup 111/In tropolone in ACD saline and ACD plasma at tropolone concentrations of 5 to 10 ..mu..g/ml, respectively, and incubation of the platelets with the tracer at room temperature for 20 minutes were optimal conditions for labeling. The authors have developed an ACD-saline kit for convenient preparation of /sup 111/In-labeled platelets. No adverse effect of /sup 111/In tropolone on platelets has been observed in studies of biodistribution, recovery, and survival of platelets in rabbits and dogs.
- Research Organization:
- Mayo Clinic, Rochester, MN
- OSTI ID:
- 6856535
- Journal Information:
- Radiology; (United States), Journal Name: Radiology; (United States) Vol. 145:1; ISSN RADLA
- Country of Publication:
- United States
- Language:
- English
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62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CHEMICAL PREPARATION
DAYS LIVING RADIOISOTOPES
DOGS
ELECTRON CAPTURE RADIOISOTOPES
INDIUM 111
INDIUM ISOTOPES
INJECTION
INTAKE
INTERMEDIATE MASS NUCLEI
INTRAVENOUS INJECTION
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
KETONES
LABELLED COMPOUNDS
LABELLING
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
RABBITS
RADIOISOTOPES
SYNTHESIS
TROPONES
VERTEBRATES