Determination of absorbed dose of ozone (O3) in animals and humans using stable-isotope (oxygen-18) tracing

Technical Report · Fri May 01 04:00:00 EDT 1987

OSTI ID:6279935

Hatch, G E; Aissa, M

A method for the determination of absorbed dose of ozone (O3) in animals and humans using oxygen-18 YO as a physiological tracer is presented. The experimental aspects of the method are based on the instantaneous pyrolysis of tissue samples and subsequent conversion of the sample oxygen to carbon monoxide then to carbon dioxide whose isotopic composition is determined by isotope-ratio mass spectrometry. A mathematical procedure is then used to correct the isotopic data for interferences from the blank and memory effects and from the iodine pentoxide oxidation of CO to CO2. Laboratory animals were exposed to YO3 (1 ppm, 1 hr) then tissues were dried and processed for YO measurement. Enrichments in YO over natural abundance YO was observed in lung homogenates, nasal cavities, trachea, and pulmonary lavage fluids but not in blood of mice, rats, and rabbits. Thus, the YO tracing method appears to be sensitive enough to detect the reaction products of YO in animals exposed to near environmental concentrations of this gas.

• Research Organization: Northrop Services, Inc., Research Triangle Park, NC (USA)
• OSTI ID: 6279935
• Report Number(s): PB-87-191706/XAB
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ABSORPTION
AIR POLLUTION
DOSES
EVEN-EVEN NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LABORATORY ANIMALS
LIGHT NUCLEI
MATHEMATICAL MODELS
NUCLEI
OXYGEN 18
OXYGEN ISOTOPES
OZONE
PHOTOCHEMICAL OXIDANTS
POLLUTION
RESPIRATION
STABLE ISOTOPES
TRACER TECHNIQUES