A method for comparison of animal and human alveolar dose and toxic effect of inhaled ozone

Journal Article · Sun Jan 01 04:00:00 EST 1989 · Health Physics; (USA)

DOI:https://doi.org/10.1097/00004032-198907001-00004· OSTI ID:7104296

Hatch, G E; Koren, H; Aissa, M ^[1]

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1. Environmental Protection Agency, Research Triangle Park, NC (USA)

Present models for predicting the pulmonary toxicity of O{sub 3} in humans from the toxic effects observed in animals rely on dosimetric measurements of O{sub 3} mass balance and species comparisons of mechanisms that protect tissue against O{sub 3}. The goal of the study described was to identify a method to directly compare O{sub 3} dose and effect in animals and humans using bronchoalveolar lavage fluid markers. The feasibility of estimating O{sub 3} dose to alveoli of animals and humans was demonstrated through assay of reaction products of {sup 18}O-labeled O{sub 3} in lung surfactant and macrophage pellets of rabbits. The feasibility of using lung lavage fluid protein measurements to quantify the O{sub 3} toxic response in humans was demonstrated by the finding of significantly increased lung lavage protein in 10 subjects exposed to 0.4 ppm O{sub 3} for 2 h with intermittent periods of heavy exercise. The validity of using the lavage protein marker to quantify the response in animals has already been established. The positive results obtained in both the {sup 18}O{sub 3} and the lavage protein studies reported here suggest that it should be possible to obtain a direct comparison of both alveolar dose and toxic effect of O{sub 3} to alveoli of animals or humans.

• OSTI ID: 7104296
• Journal Information: Health Physics; (USA), Journal Name: Health Physics; (USA); ISSN 0017-9078; ISSN HLTPA
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL MARKERS
BODY
COMPARATIVE EVALUATIONS
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
EVEN-EVEN NUCLEI
EXERCISE
INHALATION
INTAKE
ISOTOPE APPLICATIONS
ISOTOPES
LAVAGE
LIGHT NUCLEI
LUNGS
MACROPHAGES
MAMMALS
MAN
NUCLEI
ORGANS
OXYGEN 18
OXYGEN ISOTOPES
OZONE
PHAGOCYTES
PRIMATES
RABBITS
RESPIRATORY SYSTEM
SENSITIVITY
SOMATIC CELLS
STABLE ISOTOPES
TOXICITY
TRACER TECHNIQUES
VERTEBRATES