Effective ultraviolet irradiation of platelet concentrates in teflon bags

Journal Article · Mon Oct 01 04:00:00 EDT 1990 · Transfusion (Philadelphia); (USA)

DOI:https://doi.org/10.1046/j.1537-2995.1990.30891020323.x· OSTI ID:6271652

Capon, S M; Sacher, R A; Deeg, H J ^[1]

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1. Georgetown Univ., Washington, DC (USA)

Several plastic materials used in blood storage were evaluated for their ability to transmit ultraviolet B (UVB) light. A plastic bag manufactured from sheets of transparent Teflon efficiently (78-86%) transmitted UVB light and was employed in subsequent functional studies of lymphocytes and platelets exposed to UVB light while contained in these bags. In vitro experiments showed a UVB dose-dependent abrogation of lymphocyte responder and stimulator functions, with concurrent preservation of platelet aggregation responses. In a phase I pilot study, UVB-treated platelet concentrates were administered to four bone marrow transplant recipients. Adverse effects attributable to the transfusions were not observed, and patients showed clinically effective transfusion responses. No patient developed lymphocytotoxic HLA or platelet antibodies. These studies suggest that platelets can be effectively irradiated with UVB light in a closed system. However, numerous variables, including container material, volume and composition of contents, steady exposure versus agitation, and exact UV wavelength, must be considered.

• OSTI ID: 6271652
• Journal Information: Transfusion (Philadelphia); (USA), Journal Name: Transfusion (Philadelphia); (USA) Vol. 30:8; ISSN TRANA; ISSN 0041-1132
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BLOOD COAGULATION
BLOOD PLATELETS
BODY FLUIDS
BONE MARROW CELLS
CONNECTIVE TISSUE CELLS
EFFICIENCY
ELECTROMAGNETIC RADIATION
FLUORINATED ALIPHATIC HYDROCARBONS
HALOGENATED ALIPHATIC HYDROCARBONS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
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ORGANIC COMPOUNDS
ORGANIC FLUORINE COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PETROCHEMICALS
PETROLEUM PRODUCTS
PLASTICS
POLY
POLYETHYLENES
POLYTETRAFLUOROETHYLENE
PRIMATES
RADIATION EFFECTS
RADIATIONS
SOMATIC CELLS
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TEFLON
ULTRAVIOLET RADIATION
VERTEBRATES