Ultraviolet irradiation of platelet concentrates: Feasibility in transfusion practice

Journal Article · Fri Jun 01 04:00:00 EDT 1990 · Transfusion (Philadelphia); (USA)

DOI:https://doi.org/10.1046/j.1537-2995.1990.30590296370.x· OSTI ID:6606128

Andreu, G; Boccaccio, C; Lecrubier, C; Fretault, J; Coursaget, J; LeGuen, J P; Oleggini, M; Fournel, J J; Samama, M ^[1]

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1. Secteur d'Hemobiologie Transfusion, Paris (France)

Ultraviolet (UV)-B irradiation abolishes lymphocyte functions (the ability to respond and to stimulate) in mixed lymphocyte culture (MLC). This effect may have practical application in the prevention or reduction of transfusion-induced alloimmunization against HLA class I antigens. To study this, platelet concentrates (PCs) were obtained with a cell separator, suspended in autologous plasma in a final volume of 400 mL, and transferred into a large (22 X 30 cm) cell culture bag. This plastic showed a good transmittance of UV-B rays at 310 nm (54%). PCs were placed between two quartz plates (surface of irradiation = 25 X 37 cm), and the two sides were irradiated simultaneously. Energy delivered to the surface of the plastic bag was automatically monitored. The ability to respond (in MLC and to phytohemagglutinin) and to stimulate allogeneic lymphocytes was completely abolished with energy of 0.75 J per cm2 (irradiation time less than 3 min). The temperature increase during irradiation was negligible. Platelet aggregation (collagen, adrenalin, ADP, arachidonic acid, ristocetin) was not impaired if UV-B energy was below 3 J per cm2. Recovery and survival of autologous 111In-labeled platelets were studied in four volunteers; no differences were found between UV-B-treated (1.5 J/cm2) platelets and untreated platelets. These results show that a large-scale clinical trial using UV-B-irradiated PCs to prevent HLA alloimmunization is feasible.

• OSTI ID: 6606128
• Journal Information: Transfusion (Philadelphia); (USA), Journal Name: Transfusion (Philadelphia); (USA) Vol. 30:5; ISSN TRANA; ISSN 0041-1132
• Country of Publication: United States
• Language: English

Related Subjects

550301 -- Cytology-- Tracer Techniques
560120* -- Radiation Effects on Biochemicals
Cells
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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTROMAGNETIC RADIATION
ELECTRON CAPTURE RADIOISOTOPES
FEASIBILITY STUDIES
FUNCTIONS
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
PRIMATES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
SOMATIC CELLS
SURVIVAL TIME
THERAPY
TRACER TECHNIQUES
TRANSFUSIONS
ULTRAVIOLET RADIATION
VERTEBRATES