Applications of ultraviolet light in the preparation of platelet concentrates

Journal Article · Thu Jun 01 00:00:00 EDT 1989 · Transfusion (Philadelphia); (United States)

DOI:https://doi.org/10.1046/j.1537-2995.1989.29589284134.x· OSTI ID:5765522

Pamphilon, D H; Corbin, S A; Saunders, J; Tandy, N P

Passenger lymphocytes in platelet concentrates (PCs) may induce the formation of lymphocytotoxic antibodies (LCTAbs) and subsequent refractoriness to platelet transfusions. Ultraviolet (UV) irradiation can prevent lymphocytes' acting as stimulator or responder cells in mixed-lymphocyte reactions (MLRs) and could theoretically prevent LCTAb formation in vivo. A system has been devised for the delivery of UV irradiation to PCs; platelet storage characteristics and MLRs were evaluated in UV-irradiated PCs harvested from healthy donors with the Haemonetics V50 and PCS cell separators. MLR and response to phytohemagglutinin stimulation were abolished by a dose of 3000 joules per m2 at a mean wavelength of 310 nm. Platelet aggregatory responses to adenosine diphosphate (ADP), ristocetin, collagen and epinephrine, hypotonic shock response, and pH showed no important differences when control PCs and PCs irradiated as above were compared during 5 days of storage in Fenwal PL-1240 packs. Lactate production during storage was significantly higher in UV-treated PCs (p less than 0.001), but values did not exceed 20 mmol per L. UV transmission at 310 nm in standard blood product containers, including the Fenwal PL-146, PL-1240, and PL-732, was low (less than 30%), but it was acceptable in the Delmed Cryostorage and DuPont SteriCell packs (greater than 50%). UV irradiation may provide a simple and inexpensive means of producing nonimmunogenic PCs.

• Research Organization: South Western Regional Transfusion Centre, Bristol (England)
• OSTI ID: 5765522
• Journal Information: Transfusion (Philadelphia); (United States), Vol. 29:5
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTIBODY FORMATION
INHIBITION
BLOOD
STORAGE
BLOOD PLATELETS
BIOLOGICAL RADIATION EFFECTS
ADP
BLOOD COAGULATION
COLLAGEN
DOSE-RESPONSE RELATIONSHIPS
LACTATES
LEUKOCYTES
LYMPHOCYTES
MAN
TRANSFUSIONS
ULTRAVIOLET RADIATION
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACID SALTS
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
MAMMALS
MATERIALS
NUCLEOTIDES
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RADIATION EFFECTS
RADIATIONS
SCLEROPROTEINS
SOMATIC CELLS
THERAPY
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture