Evaluation of four methods for platelet compatibility testing

Journal Article · Fri May 01 04:00:00 EDT 1987 · Blood; (United States)

OSTI ID:6274843

McFarland, J G; Aster, R H

Four platelet compatibility assays were performed on serum and platelet or lymphocyte samples from 38 closely HLA-matched donor/recipient pairs involved in 55 single-donor platelet transfusions. The 22 patients studied were refractory to transfusions of pooled random-donor platelets. Of the four assays (platelet suspension immunofluorescence, PSIFT; /sup 51/Cr release; microlymphocytotoxicity; and a monoclonal anti-IgG assay, MAIA), the MAIA was most predictive of platelet transfusion outcome (predictability, 74% for one-hour posttransfusion platelet recovery and 76% for 24-hour recovery). The only other assay to reach statistical significance was the PSIFT (63% predictability for one-hour posttransfusion recovery). The degree of HLA compatibility between donor and recipient (exact matches v those utilizing cross-reactive associations) was unrelated to the ability of the MAIA to predict transfusion results. The MAIA may be capable of differentiating HLA antibodies, ABO antibodies, and platelet-specific antibodies responsible for failure of HLA-matched and selectively mismatched single-donor platelet transfusions.

• Research Organization: Blood Center of Southeastern Wisconsin, Milwaukee
• OSTI ID: 6274843
• Journal Information: Blood; (United States), Journal Name: Blood; (United States) Vol. 5; ISSN BLOOA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CHROMIUM 51
CHROMIUM ISOTOPES
DIAGNOSTIC USES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
IMMUNE REACTIONS
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
ISOTOPES
MATERIALS
MONOCLONAL ANTIBODIES
NUCLEI
PATIENTS
RADIOISOTOPES
THERAPY
TRANSFUSIONS
USES