Effects of radiofrequency radiation and simultaneous exposure with mitomycin C on the frequency of sister chromatid exchanges in Chinese hamster ovary cells

Journal Article · Thu Jan 01 04:00:00 EST 1987 · Environ. Mutagen.; (United States)

DOI:https://doi.org/10.1002/em.2860090405· OSTI ID:6271423

Ciaravino, V; Meltz, M L; Erwin, D N

Chinese hamster ovary (CHO) cells were exposed for 2 hr with and without mitomycin C (MMC) (1 X 10(-8)M) to pulsed wave radiofrequency radiation (RFR) at 2450 MHz. The repetition rate of 25,000 pulses per sec (pps), pulse width of 10 microseconds, and exposure geometry used, resulted in a specific absorption rate (SAR) of 33.8 W/kg. The following exposure regimens were used: a 37 degrees C water bath control; a water bath temperature control (TC) in which the continuously monitored medium temperature closely followed the temperature rise in the RFR-exposed flasks; and the RFR-exposed cells in a water bath set at 37 degrees C prior to exposure. RFR exposure resulted in a maximum cell culture medium temperature of 39.2 degrees C. In the absence of MMC, there was no significant increase in sister chromatid exchange (SCE) in the RFR-exposed or TC groups over that of the 37 degrees C control. When a simultaneous treatment of RFR and MMC occurred there was no statistical difference in SCE frequency from that caused by chemical treatment alone.

• Research Organization: Univ. of Texas Health Science Center, San Antonio
• OSTI ID: 6271423
• Journal Information: Environ. Mutagen.; (United States), Journal Name: Environ. Mutagen.; (United States) Vol. 4; ISSN ENMUD
• Country of Publication: United States
• Language: English

Related Subjects

560400* -- Other Environmental Pollutant Effects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
BIOLOGICAL EFFECTS
BODY
CHO CELLS
CHROMOSOMAL ABERRATIONS
DRUGS
ELECTROMAGNETIC RADIATION
FEMALE GENITALS
GENETIC EFFECTS
GONADS
MITOMYCIN
MUTATIONS
ORGANS
OVARIES
RADIATIONS
RADIOWAVE RADIATION
SISTER CHROMATID EXCHANGES