Effects of radiofrequency radiation and simultaneous exposure with mitomycin C on the frequency of sister chromatid exchanges in Chinese hamster ovary cells

Technical Report · Thu Jan 01 04:00:00 EST 1987

OSTI ID:5887211

Ciaravino, V; Meltz, M L; Erwin, D N

Chinese hamster ovary (CHO) cells were exposed for 2 hr with and without mitomycin C (MMC) to pulsed wave radiofrequency radiation (RFR) at 2450 MHz. The repetition rate of 25,000 pulses per sec (pps), and exposure geometry used, resulted in a specific absorption rate (SAR) of 33.8 W/kg. The following exposure regimens were used: 1) a 37 C water bath control; 2) a water bath temperature control (TC) in which the continuously monitored medium temperature closely followed teh temperature rise in the RFR-exposed flasks; and 3) the RFR-exposed cells in a water bath set at 37 C prior to exposure. RFR exposure resulted in a maximum cell culture medium temperature of 39.2 C. In the absence of MMC, there was no significant increase in sister chromatid exchange (SCE) in the RFR-exposed or TC groups over that of teh 37 C control. When a simultaneous treatment of RFR and MMC occurred there was no statistical difference in SCE frequency from that caused by chemical treatment alone.

• Research Organization: Texas Univ., San Antonio (USA). Health Science Center
• OSTI ID: 5887211
• Report Number(s): AD-A-184049/5/XAB
• Country of Publication: United States
• Language: English

Related Subjects

560400* -- Other Environmental Pollutant Effects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ABSORPTION
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CELL DIVISION
CHROMATIN
DRUGS
ELECTROMAGNETIC RADIATION
FEMALE GENITALS
GONADS
HAMSTERS
MAMMALS
MITOMYCIN
MITOSIS
ORGANS
OVARIES
PULSES
RADIATION EFFECTS
RADIATIONS
RADIOWAVE RADIATION
RODENTS
VERTEBRATES