Subchromosomal DNA synthesis in x-irradiated V-79 cells

Journal Article · Fri Jun 01 04:00:00 EDT 1979 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3574979· OSTI ID:6255005

Dahle, D; Griffiths, T D; Carpenter, J G

The effect of x-radiation on DNA replication in Chinese hamster V-79 cells has been investigated by DNA fiber autoradiography. The only effect observed at the replicon level is a reduction in the frequency of initiation events. The dose-response for this reduction is multiphasic indicating tha some initiation events are much more radioresistant than others. When the data obtained from DNA fiber autoradiography are compared to kinetic data for thymidine incorporation, it is evident that at least for V-79 cells, the effects of radiation of thymidine uptake into acid-precipitable material can be entirely accounted for by alterations in the frequency of initiaion of replicon clusters.

• Research Organization: Univ. of Rochester, NY
• OSTI ID: 6255005
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 78:3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CULTURES
DATA
DATA FORMS
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
HAMSTERS
INFORMATION
IONIZING RADIATIONS
IRRADIATION
ISOLATED VALUES
LABELLED COMPOUNDS
MAMMALS
NUCLEIC ACID REPLICATION
NUMERICAL DATA
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY
RODENTS
TRITIUM COMPOUNDS
VERTEBRATES
X RADIATION