Subchromosomal DNA synthesis in synchronous V-79 Chinese hamster cells after x irradiation

Journal Article · Thu Nov 01 04:00:00 EST 1984 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576348· OSTI ID:5989241

Meechan, P J; Carpenter, J G; Griffiths, T D

A substantial fraction of replicon initiation events in Chinese hamster V-79 cells have been shown to be refractory to the effects of X irradiation immediately after exposure. This study examines the possibility that the initiation radiorefractive portion is the result of changes in replicon radiosensitivity as a function of position in S phase. The data obtained from DNA fiber autoradiograms and kinetic incorporation of radiolabeled thymidine from cells irradiated at various position in S phase showed only slight changes in the proportion of replicons refractive to X irradiation immediately after exposure. These results indicate that initiation radiorefractive replicons may be an intrinsic property of V-79 cells and that cell-cycle-specific heterogeneity in radiation response cannot fully account for this phenomenon. The results also indicate that delayed inhibition of initiation events may play a larger role in the observed radiorefractive fraction than previously thought.

• Research Organization: Univ. of Rochester School of Medicine, NY
• DOE Contract Number: AC02-76EV03490
• OSTI ID: 5989241
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 100:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CARBON 14 COMPOUNDS
CELL CULTURES
CELL CYCLE
CONNECTIVE TISSUE CELLS
DNA REPLICATION
ELECTROMAGNETIC RADIATION
FIBROBLASTS
HAMSTERS
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
LABELLED COMPOUNDS
MAMMALS
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RIBOSIDES
RODENTS
SOMATIC CELLS
SURVIVAL CURVES
SYNCHRONOUS CULTURES
THYMIDINE
TRITIUM COMPOUNDS
VERTEBRATES
X RADIATION