Delayed inhibition of subchromosomal DNA synthesis in V-79 Chinese hamster cells after. gamma. irradiation

Journal Article · Thu Nov 01 04:00:00 EST 1984 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576349· OSTI ID:6152441

Meechan, P J; Griffiths, T D

Existence of a substantial fraction of replicon initiation events refractory to the effects of X irradiation in Chinese hamster cells has been reported by several laboratories. The work reported here examined whether this apparently refractive fraction resulted from a delayed inhibition of initiation events. Data obtained from velocity sedimentation studies indicated that the extent of inhibition increased over the first hour after irradiation from 35% inhibition immediately following exposure to 3 kR to 75% inhibition of initiation 1 hr after irradiation. Analysis of subsequent recovery of initiation radiosensitivity was performed using DNA fiber autoradiograms prepared from cells incubated up to 4 hr between 2-kR exposures. The data from these experiments indicated that some recovery occurs within 1 hr of irradiation and thus separation of the inhibition and recovery processes in V-79 cells may not be feasible.

• Research Organization: Univ. of Rochester School of Medicine, NY
• OSTI ID: 6152441
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 100:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
CARBON 14 COMPOUNDS
COBALT 60
COBALT ISOTOPES
CONNECTIVE TISSUE CELLS
DATA
DNA
DNA REPLICATION
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
FIBROBLASTS
GAMMA RADIATION
HAMSTERS
INFORMATION
INHIBITION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUMERICAL DATA
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RECOVERY
RODENTS
SOMATIC CELLS
TRITIUM COMPOUNDS
VERTEBRATES
X RADIATION
YEARS LIVING RADIOISOTOPES