Comparison of the properties of NTPase in avian myeloblastosis virus and myeloblasts

Technical Report · Wed Sep 01 04:00:00 EDT 1982

OSTI ID:6251304

Schneider, R P

Subviral particles containing viral nucleoside triphosphatase (NTPase) activity were isolated from sucrose density gradients of avian myeloblastosis virus (AMV) exposed to 1% cholate at 0/sup 0/ for 2 hr. The NTPase fraction contained about 10% of the protein added to the gradients; the remainder of the protein was soluble, demonstrating that virus cores were disrupted. Specific activity of the NTPase in the isolated preparation was threefold higher than that of AMV and sevenfold higher than cholate-exposed AMV. Gel electrophoresis in sodium dodecyl sulfate revealed that the particles contained at least nine proteins, including the major and minor viral glycoproteins; only traces of core proteins were present. Electron microscopy of negative-stained preparations demonstrated that the material was, for the most part, aggregates of AMV-sized vesicles, suggesting that the preparation consisted of isolated virus envelopes. Intact myeloblasts exhibited the NTPase activity on their external surfaces. The specific activity of a membrane fraction isolated from these cells was about six times less than that of a virion and 25 times less than that of a viral envelope fraction. The data suggest that the nascent envelope accumulates the enzyme during its formation in the cell membrane. 31 references, 6 figures, 4 tables.

• Research Organization: Pacific Northwest Lab., Richland, WA (USA)
• DOE Contract Number: AC06-76RL01830
• OSTI ID: 6251304
• Report Number(s): PNL-SA-10809; ON: DE85006085
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIRDS
CELL CONSTITUENTS
CELL MEMBRANES
DATA
ENZYME ACTIVITY
ENZYMES
ESTERASES
EXPERIMENTAL DATA
HYDROLASES
INFORMATION
MEMBRANES
MICROORGANISMS
NUMERICAL DATA
ORGANIC COMPOUNDS
PARASITES
PHOSPHATASES
PROTEINS
SOMATIC CELLS
STEM CELLS
VERTEBRATES
VIRUSES