Enzymatic radioiodination of the envelope proteins of avian myeloblastosis virus

Journal Article · Tue Jan 01 04:00:00 EST 1974 · J. Virol., v. 13, no. 1, pp. 42-45

OSTI ID:4408930

Fritz, R B

Purified avian myeloblastosis virus was iodinated by the lactoperoxidase method. Disruption of the labeled virions and chromatography of the viral proteins showed that radioiodine was associated only with the glycoproteins of the viral envelope. Reaction of the radiolabeled virns with antiviral antibody followed by mild detergent treatment and subsequent recovery of immune complexes showed this technique to be useful for the isolation of viral envelope antigens. (auth)

• Research Organization: Emory Univ., Atlanta
• NSA Number: NSA-29-010498
• OSTI ID: 4408930
• Journal Information: J. Virol., v. 13, no. 1, pp. 42-45, Journal Name: J. Virol., v. 13, no. 1, pp. 42-45; ISSN JOVIA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

*IODINE 125-- RADIOCHEMISTRY
*PROTEINS-- IODINATION
*VIRUSES-- IODINATION
ANTIBODIES
ANTIGEN-ANTIBODY REACTIONS
CHROMATOGRAPHY
GLUCOPROTEINS
LABELLING
N40420 --Chemistry--Radiochemistry & Nuclear Chemistry--Properties of Radioactive Materials
N48740* --Life Sciences--Nuclide Kinetics & Toxicology-- Microorganisms
PEROXIDASES