Quantitation of avian RNA tumor virus reverse transcriptase by radioimmunoassay

Journal Article · Tue Jul 01 04:00:00 EDT 1975 · J. Virol., v. 16, no. 1, pp. 146-152

OSTI ID:4175390

Panet, A; Baltimore, D; Hanafusa, T

A radioimmunoassay was developed that can detect and quantitate 3 ng or more of the avian RNA tumor virus reverse transcriptase. The assay detected no antigenic sites in Rous sarcoma virus $alpha$ virions or in virions of a murine RNA tumor virus. About 70 molecules of reverse transcriptase were found per virion of avian myeloblastosis virus with this assay or with an assay based on antibody inhibition of enzymatic activity. The assay detected about 270 ng of enzyme per mg of cell protein in virus-producing cells; uninfected cells had much less antigenic material but contained some determinants able to displace radioactive antigen. No additional antigenic determinants on reverse transcriptase could be detected that were not found on the separated $alpha$ subunit of the enzyme. Although sevenfold less sensitive than enzymatic activity as a measure of reverse transcriptase, the radioimmunoassay can detect antigen using small amounts of protein and in the presence of inhibitors. (auth)

• Research Organization: Massachusetts Inst. of Tech., Cambridge
• NSA Number: NSA-33-005882
• OSTI ID: 4175390
• Journal Information: J. Virol., v. 16, no. 1, pp. 146-152, Journal Name: J. Virol., v. 16, no. 1, pp. 146-152; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

*ENZYMES-- RADIOIMMUNOASSAY
*VIRUSES-- ENZYMES
550601* --Biomedical Sciences
Basic Studies--Medicine-- Unsealed Radionuclides in Diagnostics
IODINE 125
N48830* --Life Sciences--New Tracer Techniques--Other
NEOPLASMS
RNA
TRACER TECHNIQUES