Envelope proteins of bovine herpesvirus 1: immunological and biochemical studies

Thesis/Dissertation · Wed Jan 01 04:00:00 EST 1986

OSTI ID:6614113

Rodriguez Roque, L L

The authors studied immunological and biochemical properties of the bovid herpesvirus 1 (BHV-1) envelope proteins in order to understand the pathogenesis of BHV-1 infection and to provide basic information for the production of effective subunit vaccines against BHV-1. Ten glycoproteins MW 180, 150, 130, 115, 97, 77, 74, 64, 55, and 45 kilodaltons (K), and a single non-glycosylated 108 K protein were quantitatively removed from purified BHV-1 virions by detergent treatment. These glycoproteins were present on the virion envelope and on the surface of BHV-1 infected cells. The quantitative removal from virions by treatment with nonionic detergents and their presence on the surface of infected cells indicate that 180/97, 150/77, and 130/74/55 K are major components of the BHV-1 envelope and are also the targets of virus neutralizing humoral immune response. Envelope glycoproteins of herpes simplex type 1 (HSV-1) bind immunoglobulin by the Fc end and it is suggested this may increase pathogenicity of this virus. They searched for a similar function in BVH-1 by measuring the ability of BHV-1 infected cells and viral envelope proteins to bind radiolabelled rabbit and bovine IgG. Binding activity for rabbit IgG or bovine IgG-Fc could not be demonstrated by BHV-1 infected MDBK cells, whereas, MDBK cells infected with HSV-1 bound rabbit IgG and bovine IgG-Fc. None of the three major envelope proteins of BHV-1 bound to rabbit or bovine IgG. The results of this study indicate that BHV-1, unlike some other herpesviruses, lack Fc binding activity.

• Research Organization: Wisconsin Univ., Madison (USA)
• OSTI ID: 6614113
• Country of Publication: United States
• Language: English

Related Subjects

550701* -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMISTRY
CARBOHYDRATES
CHEMISTRY
DISEASES
GLOBULINS
GLUCOPROTEINS
HERPES SIMPLEX
IMMUNOGLOBULINS
INFECTIOUS DISEASES
LABELLED COMPOUNDS
MICROORGANISMS
ORGANIC COMPOUNDS
PARASITES
PATHOGENESIS
PROTEINS
SACCHARIDES
SKIN DISEASES
VACCINES
VIRAL DISEASES
VIRUSES