Herpes simplex viruses lacking glycoprotein D are unable to inhibit virus penetration: quantitative evidence for virus-specific cell surface receptors
Herpes simplex virus (HSV) glycoprotein D (gD) plays an essential role in the entry of virus into cells. HSV mutants unable to express gD were constructed. The mutants can be propagated on VD60 cells, which supply the viruses with gD; however, virus particles lacking gD were produced in mutant-infected Vero cells. Virus particles with or without gD adsorbed to a large number of sites on the cell surface; however, virions lacking gD did not enter cells. Cells pretreated with UV-inactivated virions containing gD were resistant to infection with HSV type 1 (HSV-1) and HSV-2. In contrast, cell pretreated with UV-inactivated virions lacking gD could be infected with HSV-1 and HSV-2. If infectious HSV-1 was added prior to UV-inactivated virus particles containing gD, the infectious virus entered cells and replicated. Therefore, virus particles containing gD appear to block specific cell surface receptors which are very limited in number. Particles lacking gD are presumably unable to interact with these receptors, suggesting that gD is an essential receptor-binding polypeptide.
- Research Organization:
- McMaster Univ., Hamilton, Ontario (Canada)
- OSTI ID:
- 6399081
- Journal Information:
- J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 62:12; ISSN JOVIA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOCHEMISTRY
CARBOHYDRATES
CHEMISTRY
DISEASES
ELECTROMAGNETIC RADIATION
EXTREME ULTRAVIOLET RADIATION
GLUCOPROTEINS
GLYCOPROTEINS
HERPES SIMPLEX
INFECTIOUS DISEASES
INHIBITION
MAMMALS
MAN
MEMBRANE PROTEINS
MICROORGANISMS
MONOCLONAL ANTIBODIES
MUTATIONS
ORGANIC COMPOUNDS
PARASITES
PRIMATES
PROTEINS
RADIATIONS
RECEPTORS
SACCHARIDES
SKIN DISEASES
ULTRAVIOLET RADIATION
VERTEBRATES
VIRAL DISEASES
VIRUSES