Quantitative assay for transformation of 3T3 cells by herpes simplex virus type 2

Journal Article · Sat Mar 01 04:00:00 EDT 1975 · J. Virol., v. 15, no. 3, pp. 490-496

OSTI ID:4168524

Duff, R; Rapp, F

The interaction of herpes simplex virus type 1 (HSV-1) and type 2 (HSV- 2) with Swiss/3T3 cells was investigated. Virus-induced cytopathic effects developed in the absence of production of infectious virus. HSV-2 inactivated with uv light (2, 4, 6, and 8 min) also induced cell death in the absence of virus replication. Cell death was not detectable after infection by HSV-2 that had been inactivated by uv irradiation for 10, 12, and 14 min. 3T3 cells infected with uv-inactivated virus (10 and 12 min) continued to replicate past the contact-inhibited monolayer normally associated with these cells. Infection of 3T3 cells with uv-irradiated HSV-2 also induced the development of transformed foci. Transformed cells with an epithelioid or fibroblastoid morphology were identified and isolated. All HSV-2-transformed cell lines contained HSV-2- specific antigens detectable by immunofluorescence techniques. The maximum frequency of HSV-2-induced transformation was 3 x 10$sup 5$ PFU per transformed focus, and the observed transformation could be inhibited by pretreatment of the virus with specific antiserum. No type C particles were detected within five cell culture passages after transformation by HSV-2. Type C virus particles were detected after 10 cell culture passages of the HSV-2-transformed cell lines. (auth)

• Research Organization: Pennsylvania State Univ., Hershey
• NSA Number: NSA-33-005718
• OSTI ID: 4168524
• Journal Information: J. Virol., v. 15, no. 3, pp. 490-496, Journal Name: J. Virol., v. 15, no. 3, pp. 490-496; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

*ANIMAL CELLS-- BIOASSAY
*HERPES SIMPLEX
*VIRUSES-- INACTIVATION
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CELL KILLING
N48310* --Life Sciences--Radiation Effects on Microorganisms- -Basic Studies
ULTRAVIOLET RADIATION