Early inhibition of cellular CNA synthesis by high multiplicities of infectious and uv-inactivated reovirus

Journal Article · Mon Oct 01 04:00:00 EDT 1973 · J. Virol., v. 12, no. 4, pp. 704-710

OSTI ID:4437873

Shaw, J E; Cox, D C

Inhibition of cellular DNA synthesis began 6 to 8 h after reovirus infection at a multiplicity of infection of 10 PFU per cell. However, as the multiplicity of infection was increased to a maximum of 10/sup 3/ PFU/cell, inhibition of DNA synthesis began earlier after infection (2 to 4 h postinfection), and the initial rate of inhibition increased. The enhanced inhibition of DNA replication at high virus multiplicities appeared to be selective since RNA synthesis was not detectably altered as late as 9 h postinfection and inhibition of protein synthesis did not begin until 7 to 9 h after infection. Early inhibition of DNA synthesis did not appear to be related to changes in thymidine pool characteristics, thymidine kinase activity, or detectable degradation of cellular DNA. Even though the particleto-PFU ratio was increased by ultraviolet light inactivation of virus, the ability to induce early inhibition of DNA synthesis was not diminished. (auth)

• Research Organization: Univ. of Oklahoma, Norman
• NSA Number: NSA-29-000555
• OSTI ID: 4437873
• Journal Information: J. Virol., v. 12, no. 4, pp. 704-710, Journal Name: J. Virol., v. 12, no. 4, pp. 704-710; ISSN JOVIA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

*DNA-- BIOSYNTHESIS
INHIBITION
N48310* --Life Sciences--Radiation Effects on Microorganisms- -Basic Studies
PHOSPHOTRANSFERASES
PHOTON BEAMS
PROTEINS
THYMIDINE
ULTRAVIOLET RADIATION