Focus formation and neoplastic transformation by herpes simplex virus type 2 inactivated intracellularly by 5-bromo-2'-deoxyuridine and near UV light

Journal Article · Thu Jan 01 04:00:00 EST 1981 · J. Virol.; (United States)

OSTI ID:5672734

Manak, M M; Aurelian, L; Ts'o, P O

The induction of focus formation in low serum and of neoplastic transformation of Syrian hamster embryo cells was examined after the expression of herpes simplex virus type 2 functions. Syrian hamster embryo cells infected at a high multiplicity (5 PFU/cell) with 5-bromo-2'-deoxyuridine-labeled herpes simplex virus type 2 (11% substitution of thymidine residues) were exposed to near UV light irradiation at various times postinfection. This procedure specifically inactivated the viral genome, while having little, if any, effect on the unlabeled cellular DNA. Focus formation in 1% serum and neoplastic transformation were observed in cells exposed to virus inactivated before infection, but the frequency was enhanced (15- to 27-fold) in cells in which the virus was inactivated at 4 to 8 h postinfection. Only 2 to 45 independently isolated foci were capable of establishing tumorigenic lines. The established lines exhibited phenotypic alterations characteristic of a transformed state, including reduced serum requirement, anchorage-independent growth, and tumorigenicity. They retained viral DNA sequences and, even at relatively late passage, expressed viral antigens, including ICP 10.

• Research Organization: Department of Comparative Medicine The Johns Hopkins Medical Institutions, Baltimore, Maryland
• OSTI ID: 5672734
• Journal Information: J. Virol.; (United States), Journal Name: J. Virol.; (United States) Vol. 40:1; ISSN JOVIA
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIMETABOLITES
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BROMOURACILS
BUDR
CONNECTIVE TISSUE CELLS
DISEASES
DRUGS
ELECTROMAGNETIC RADIATION
EMBRYONIC CELLS
FIBROBLASTS
HAMSTERS
HERPES SIMPLEX
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IN VITRO
INACTIVATION
INFECTIOUS DISEASES
MAMMALS
NEAR ULTRAVIOLET RADIATION
NUCLEOSIDES
NUCLEOTIDES
ONCOGENIC TRANSFORMATIONS
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RIBOSIDES
RODENTS
SKIN DISEASES
SOMATIC CELLS
ULTRAVIOLET RADIATION
URACILS
VERTEBRATES
VIRAL DISEASES