Double-labelled HIV-1 particles for study of virus-cell interaction

Journal Article · Fri Mar 30 04:00:00 EDT 2007 · Virology

DOI:https://doi.org/10.1016/j.virol.2006.10.005· OSTI ID:20977002

Lampe, Marko ^[1]; Briggs, John A.G. ^[2]; Endress, Thomas ^[2]; Glass, Baerbel ^[1]; Riegelsberger, Stefan ^[2]; Kraeusslich, Hans-Georg ^[1]; Lamb, Don C ^[3]; Braeuchle, Christoph ^[3]; Mueller, Barbara ^[1]

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1. Department of Virology, Universitaetsklinikum Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg (Germany)
2. Department of Chemistry and Biochemistry, Ludwig-Maximilians-Universitaet Muenchen, Butenandtstr. 11, Haus E, 81377 Munich (Germany)
3. Center for NanoScience, Ludwig-Maximilians-Universitaet Muenchen, Geschwister-Scholl-Platz 1, 80539 Munich (Germany)

Human immunodeficiency virus (HIV) delivers its genome to a host cell through fusion of the viral envelope with a cellular membrane. While the viral and cellular proteins involved in entry have been analyzed in detail, the dynamics of virus-cell fusion are largely unknown. Single virus tracing (SVT) provides the unique opportunity to visualize viral particles in real time allowing direct observation of the dynamics of this stochastic process. For this purpose, we developed a double-coloured HIV derivative carrying a green fluorescent label attached to the viral matrix protein combined with a red label fused to the viral Vpr protein designed to distinguish between complete virions and subviral particles lacking MA after membrane fusion. We present here a detailed characterization of this novel tool together with exemplary live cell imaging studies, demonstrating its suitability for real-time analyses of HIV-cell interaction.

• OSTI ID: 20977002
• Journal Information: Virology, Journal Name: Virology Journal Issue: 1 Vol. 360; ISSN VIRLAX; ISSN 0042-6822
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
AIDS VIRUS
CELL MEMBRANES
FLUORESCENCE
LABELLING
PROTEINS
STOCHASTIC PROCESSES
SULFATES