Four kits for plasma digoxin radioimmunoassay compared. [/sup 86/Rb tracer technique]

Journal Article · Tue Feb 01 04:00:00 EST 1977 · Clin. Chem.; (United States)

OSTI ID:6228192

Bergdahl, B ^[1]; Molin, L; Lindwall, L; Dahlstroem, G; Scherling, I L; Bertler, A

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1. University Hospital, Linkoeping, Sweden

We evaluated four commercial radioimmunoassay kits for digoxin. We assayed a standard plasma containing digoxin, 2.0 ..mu..g/L, and samples from patients receiving digoxin, with use of the kits and of a bioassay, the /sup 86/Rb-uptake inhibition technque. Intra-assay precisions differed significantly. Computer-calculated 95% confidence intervals for the radioimmunoassays averaged 0.4 to 0.6 ..mu..g/L at the proposed toxic threshold of 2.0 ..mu..g/L; the corresponding value of the /sup 86/Rb assay was 0.75 ..mu..g/L. Digoxin in the standard plasma was overestimated with three of the kits (means: 2.40, 2.56, and 2.59 ..mu..g/L) but was assayed accurately by the /sup 86/Rb technique and by one kit. This same kit gave a significantly lower mean (1.07 ..mu..g/L) for the patients' samples then did the other three kits (1.32, 1.49, and 1.29 ..mu..g/L), two of which also differed significantly in accuracy. The /sup 86/Rb assay measured glycoside activity corresponding to a mean digoxin concentration of 1.35 ..mu..g/L. We conclude that the relatively low precision of digoxin assay and the variations in accuracy between kits from various vendors apparently deserve continual attention.

• OSTI ID: 6228192
• Journal Information: Clin. Chem.; (United States), Journal Name: Clin. Chem.; (United States) Vol. 25:2; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALKALI METAL ISOTOPES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD PLASMA
BODY FLUIDS
CARBOHYDRATES
CARDIOTONICS
COMPARATIVE EVALUATIONS
DATA
DATA FORMS
DAYS LIVING RADIOISOTOPES
DIGITALIS GLYCOSIDES
DIGOXIN
DRUGS
EVALUATION
EXPERIMENTAL DATA
GLYCOSIDES
GRAPHS
INFORMATION
INTERMEDIATE MASS NUCLEI
ISOLATED VALUES
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUMERICAL DATA
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PATIENTS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
RUBIDIUM 86
RUBIDIUM ISOTOPES
STEROIDS
TRACER TECHNIQUES