Variability among commercially available digoxin radioimmunoassay kits in cross reactivity to dihydrodigoxin. [/sup 125/I]

Journal Article · Sun Jan 01 04:00:00 EST 1978 · Clin. Chem.; (United States)

OSTI ID:5734377

Kramer, W G; Kinnear, N L; Morgan, H K

We evaluated four commercially available /sup 125/I-digoxin radioimmunoassay kits with regard to their ability to cross react with the digoxin metabolite dehydrodigoxin. We prepared dihydrodigoxin serum samples in digoxin-free serum over the concentration range 0.4 to 5.0 microgram/liter and assayed them with each kit according to the manufacturer's instructions. The metabolite was able to displace the /sup 125/I-labeled digoxin derivative from the antibody supplied with all four kits. However, the extent of the cross reactivity depended on the kit, ranging from essentially zero to a high degree of interference. Dihydrodigoxin is the only metabolite of digoxin to have been quantitated in human serum, and may comprise up to 30% of total glycosides. Over the clinical and therapeutic range of serum digoxin concentrations, enough dihydrodigoxin can be produced to interfere in the determination of serum digoxin concentrations by this method. We suggest that laboratories evaluate their specific kit with regard to cross reactivity to this metabolite.

• Research Organization: Univ. of Houston, TX
• OSTI ID: 5734377
• Journal Information: Clin. Chem.; (United States), Journal Name: Clin. Chem.; (United States) Vol. 24:1; ISSN CLCHA
• Country of Publication: United States
• Language: English

Related Subjects

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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIGEN-ANTIBODY REACTIONS
BETA DECAY RADIOISOTOPES
CARBOHYDRATES
CARDIOTONICS
COMPARATIVE EVALUATIONS
DAYS LIVING RADIOISOTOPES
DIGITALIS GLYCOSIDES
DIGOXIN
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
GLYCOSIDES
IMMUNE SERUMS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MEDICAL SUPPLIES
METABOLITES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
STEROIDS
TRACER TECHNIQUES