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Effect of low dose rate irradiations with fission-spectrum neutrons on mutation induction of Chinese hamster cells and human diploid fibroblasts

Technical Report ·
OSTI ID:6212759
; ;

Cell survival and induction of the forward mutation leading to 6-thioguanine resistance were measured in Chinese hamster V79 cells and human diploid lung fibroblast 1MR-91 cells exposed to fission-spectrum neutrons (from the JANUS reactor at the Argonne National Laboratory) delivered at both high and reduced dose rates. Exponentially growing cells were irradiated at 10.3 or 20.6 cGy/min for high dose rate and at 0.43 cGy/min for reduced dose rate. Both cell lines exposed to neutrons showed little or no significant differences in cell killing between acute and protracted doses. On the other hand, mutation induction of V79 cells after low dose rate exposures to fission-spectrum neutrons, given at 0.43 cGy/min, was significantly enhanced at doses above 100 cGy as compared to that after high dose rate exposures. Protracted irradiation of IMR-91 cells with neutrons also resulted in enhancement of the mutation frequency in the dose region of up to 75 cGy. These results are discussed in terms of cell cycle dependency for mutation induction and error-prone repair of mutational damage after protracted irradiations with fission-spectrum neutrons. 33 refs., 5 figs.

Research Organization:
Argonne National Lab., IL (USA)
DOE Contract Number:
W-31109-ENG-38
OSTI ID:
6212759
Report Number(s):
ANL/PPRNT-89-153; ON: DE89008452
Country of Publication:
United States
Language:
English

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Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BEAMS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOTECHNOLOGY
CELL CULTURES
CHRONIC EXPOSURE
CHRONIC IRRADIATION
DNA REPAIR
DOSE RATES
HAMSTERS
IRRADIATION
LOW DOSE IRRADIATION
MAMMALS
MAN
MUTATIONS
NEUTRON BEAMS
NUCLEON BEAMS
PARTICLE BEAMS
PRIMATES
RADIOINDUCTION
RECOVERY
REPAIR
RODENTS
VERTEBRATES