Morphological transformation of Syrian hamster embryo cells by low doses of fission neutrons delivered at different dose rates

Technical Report · Tue Jan 01 04:00:00 EST 1991

DOI:https://doi.org/10.2172/5704550· OSTI ID:5704550

Jones, C A; Sedita, B A ^[1]; Hill, C K ^[2]; Elkind, M M ^[3]

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1. Argonne National Lab., IL (USA)
2. University of Southern California, Los Angeles, CA (USA). Cancer Research Lab.
3. Colorado State Univ., Fort Collins, CO (USA). Dept. of Radiology and Radiation Biology

Both induction of cell transformation and killing were examined with Syrian hamster embryo (SHE) fibroblasts exposed to low doses of JANUS fission-spectrum neutrons delivered at high (10.3 cGy/min) and low (0.43 and 0.086 cGy/min) dose rates. Second-passage cells were irradiated in mass cultures, then cloned over feeder cells. Morphologically transformed colonies were identified 8-10 days later. Cell killing was independent of dose rate, but the yield of transformation was greater after low-dose-rate irradiations. Decreasing the neutron dose-rate from 10.3 to 0.086 cGy/min resulted in a two- to threefold increase in the yield of transformation for neutron exposures below 50 cGy, and enhancement which was consistently observed in repetitive experiments in different radiosensitive SHE cell preparations. 43 refs., 5 figs., 1 tab.

• Research Organization: Argonne National Lab., IL (USA)
• Sponsoring Organization: DOE; DOHHS; USDOE, Washington, DC (USA); Department of Health and Human Services, Washington, DC (USA)
• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 5704550
• Report Number(s): ANL/PP-67439; ON: DE91011113; CNN: RO1CA33974; CA47497
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BARYONS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL KILLING
CELL TRANSFORMATIONS
DOSES
ELEMENTARY PARTICLES
EMBRYONIC CELLS
FERMIONS
FISSION NEUTRONS
HADRONS
HAMSTERS
IRRADIATION
LOW DOSE IRRADIATION
MAMMALS
MORPHOLOGICAL CHANGES
NEUTRONS
NUCLEONS
RADIATION DOSES
RADIATION EFFECTS
RADIOSENSITIVITY
RBE
RODENTS
VERTEBRATES