Effects of low-dose radiation on gene expression in Syrian hamster embryo cells: Comparison of JANUS neutrons and gamma rays

Conference · Wed Jul 01 04:00:00 EDT 1992

OSTI ID:10155018

Woloschak, G E; Chang-Liu, C M

Past work by or group and others has shown the modulation of specific genes following exposure of cells to ionizing radiation. Many classes of genes have been found to be modulated in response to ionizing radiation, including those encoding cytoskeletal elements, cell growth arresting proteins, cytokines, and cellular oncogenes. The functions of this specific modulation of gene expression are currently being investigated by several groups: it has been suggested that gene modulation in response to radiation plays a role in the cellular repair of DNA damage, cell survival, or cellular transformation. Several groups have examined induction of nuclear proto-oncogenes following exposure to DNA-damaging agents. In all experiments, we examined modulation of gene expression by ionizing radiations in Syrian hamster embryo (SHE) fibroblasts, which are normal diploid cells that can be neoplastically transformed by low doses of ionizing radiations. Cells plated in 100-mm Petri plates containing 10 ml of medium were irradiated with {sup 60}C {gamma}-rays or fission-spectrum neutrons (0.85 MeV) from the JANUS reactor. All irradiations were performed at 37{degrees}C on cycling cells; equitoxic doses of neutrons and {gamma}-rays were selected on the basis of survival data.

• Research Organization: Argonne National Lab., IL (United States)
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 10155018
• Report Number(s): ANL/CP--75218; CONF-920720--1; ON: DE92016176
• Country of Publication: United States
• Language: English

Related Subjects

550400
560120
560150
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
DNA
DOSE RATES
EMBRYOS
GENETIC RADIATION EFFECTS
GENETICS
HAMSTERS
IONIZING RADIATIONS
PROTEINS
RADIATION EFFECTS ON ANIMALS
RADIATION EFFECTS ON BIOCHEMICALS, CELLS, AND TISSUE CULTURE
RNA