Modulation of expression of genes encoding nuclear proteins following exposure to JANUS neutrons or {gamma}-rays

Technical Report · Sun May 01 04:00:00 EDT 1994

DOI:https://doi.org/10.2172/10148904· OSTI ID:10148904

Woloschak, G E ^[1]; Chang-Liu, Chin-Mei ^[2]

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1. Loyola Univ. Medical Center, Maywood, IL (United States)
2. Argonne National Lab., IL (United States)

Previous work has shown that exposure of cells to ionizing radiations causes modulation of a variety of genes, including those encoding c-fos, interleukin-1, tumor necrosis factor, and cytoskeletal elements. The experiments reported herein were designed to examine the effects of either JANUS neutron or {gamma}-ray exposure on expression of genes encoding nucleus-associated proteins (H4-histone, c-jun, c-myc, Rb, and p53). Cycling Syrian hamster embryo cells were irradiated with varying doses and dose rates of either JANUS fission-spectrum neutrons or {gamma}-rays; after incubation of the cell cultures for 1 h following radiation exposure, mRNA was harvested and analyzed by Northern blot. Results revealed induction of transcripts for c-jun, H4-histone, and (to a lesser extent) Rb following {gamma}-ray but not following neutron exposure. Expression of p53 and c-myc genes was unaffected by radiation exposure. Radiations at different doses and dose rates were compared for each of the genes studied.

• Research Organization: Argonne National Lab., IL (United States)
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 10148904
• Report Number(s): ANL/BIM/PP--78237; ON: DE94011487
• Country of Publication: United States
• Language: English

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RADIOBIOLOGY