Differential modulation of specific gene expression following high- and low-LET radiations

Journal Article · Thu Nov 01 04:00:00 EST 1990 · Radiation Research; (USA)

DOI:https://doi.org/10.2307/3577864· OSTI ID:6168120

Woloschak, G E; Chang-Liu, C M ^[1]

---

1. Argonne National Laboratory, IL (USA)

Experiments were designed to examine the effects of radiation quality on specific gene expression within the first 3 h following radiation exposure in Syrian hamster embryo (SHE) cells. Preliminary work demonstrated the induction of c-fos and alpha-interferon genes following exposure to low-linear-energy-transfer (low-LET) radiations (X rays or gamma rays). More detailed experiments revealed induction of c-fos mRNA within the first 3 h following exposure to either X rays (75 cGy) or gamma rays (90 cGy). We could not detect induction of c-fos following exposure of SHE cells to fission-spectrum neutrons (high-LET) from the JANUS reactor administered at either high (12 cGy/min) or low (0.5 cGy/min) dose rates. Expression of alpha-interferon mRNA was similarly induced by low-LET radiations but only modestly by JANUS neutrons. The induction by gamma rays was dose-dependent, while induction by neutrons was specific for low doses and low dose rates. These experiments demonstrate the differential gene inductive response of cells following exposure to high- and low-LET radiations. These experiments suggest that these different qualities of ionizing radiation may have different mechanisms for inducing many of the cellular consequences of radiation exposure, such as cell survival and cell transformation.

• OSTI ID: 6168120
• Journal Information: Radiation Research; (USA), Journal Name: Radiation Research; (USA) Vol. 124:2; ISSN RAREA; ISSN 0033-7587
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ABSCOPAL RADIATION EFFECTS
ANIMAL CELLS
BARYONS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CHO CELLS
CONNECTIVE TISSUE CELLS
DOSES
ELECTROMAGNETIC RADIATION
ELEMENTARY PARTICLES
ENERGY TRANSFER
FERMIONS
FIBROBLASTS
FISSION NEUTRONS
GAMMA RADIATION
GENE REGULATION
GENES
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
GROWTH FACTORS
HADRONS
IN VITRO
INTERFERON
IONIZING RADIATIONS
LET
LYMPHOKINES
MESSENGER-RNA
MITOGENS
NEUTRONS
NUCLEIC ACIDS
NUCLEONS
ONCOGENES
ORGANIC COMPOUNDS
PROTEINS
RADIATION DOSES
RADIATION EFFECTS
RADIATIONS
RNA
SOMATIC CELLS
X RADIATION