Low doses of neutrons induce changes in gene expression

Conference · Fri Jan 01 04:00:00 EST 1993

OSTI ID:6330551

Woloschak, G E; Chang-Liu, C M ^[1]; Panozzo, J; Libertin, C R ^[2]

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1. Argonne National Lab., IL (United States)
2. Loyola Univ., Maywood, IL (United States)

Studies were designed to identify genes induced following low-dose neutron but not following [gamma]-ray exposure in fibroblasts. Our past work had shown differences in the expression of [beta]-protein kinase C and c-fos genes, both being induced following [gamma]-ray but not neutron exposure. We have identified two genes that are induced following neutron, but not [gamma]-ray, exposure: Rp-8 (a gene induced by apoptosis) and the long terminal repeat (LTR) of the human immunodeficiency (HIV). Rp-8 mRNA induction was demonstrated in Syrian hamster embryo fibroblasts and was found to be induced in cells exposed to neutrons administered at low (0.5 cGy/min) and at high dose rate (12 cGy/min). The induction of transcription from the LTR of HIV was demonstrated in HeLa cells bearing a transfected construct of the chloramphenicol acetyl transferase (CAT) gene driven by the HIV-LTR promoter. Measures of CAT activity and CAT transcripts following irradiation demonstrated an unresponsiveness to [gamma] rays over a broad range of doses. Twofold induction of the HIV-LTR was detected following neutron exposure (48 cGy) administered at low (0.5 cGy/min) but not high (12 cGy/min) dose rates. Ultraviolet-mediated HIV-LTR induction was inhibited by low-dose-rate neutron exposure.

• Research Organization: Argonne National Lab., IL (United States)
• Sponsoring Organization: DOE; USDOE, Washington, DC (United States)
• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 6330551
• Report Number(s): ANL/BIM/CP-79252; CONF-9305202--1; ON: DE93015146
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AIDS VIRUS
ANIMAL CELLS
BARYONS
BIOLOGY
CELL CULTURES
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
ELEMENTARY PARTICLES
ENZYME INDUCTION
FERMIONS
FIBROBLASTS
GAMMA RADIATION
GENE OPERONS
GENE REGULATION
HADRONS
HYDROGEN COMPOUNDS
IONIZING RADIATIONS
ISOTOPE APPLICATIONS
MICROORGANISMS
NEUTRONS
NUCLEONS
PARASITES
RADIATIONS
RADIOBIOLOGY
SOMATIC CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
VIRUSES